Cell-based Therapies

The group around Susanne Wolbank is examining a variety of cells to study their potential use in cell-based therapies. This includes for example the isolation and cultivation of primary cells from animal or human tissue sources as well as preparing them for application in regenerative medicine. An emphasis is put on different parts of the cell with the cell organelles but also on proliferation and the communication within cell bonds as well as the characteristics and functionality of specific cell types. Researching mechanisms on a cellular level plays an important role in order to clarify and better understand cellular structures. The group works on various different projects and provides cells for the different research areas in tissue regeneration. This includes for example bone-, ligament-, cartilage-, tendon- and neuro-regeneration but also the endothelium and light therapy group. The focus is put on the following technical methods:

  • Expansion and marker characterisation
  • Cytotoxicity assays
  • 3D culture
  • Differentiation assays
  • Transfection
  • Reporter assays

One emphasis is put on using “clinical waste material”. This includes for example using the human placenta and its different components such as the amniotic membrane. From the amnion, adult stem cells can be isolated and cultivated to be used in regenerative medicine. Other human “waste material” accumulates during liposuction. Thereby, the various cells originating from adipose tissue can be used again in tissue regeneration.

a. Epithelial layer of intact human amniotic membrane, stained with tetramethylrhodamine methyl ester (TMRM) and MitoTracker Green FM for mitochondrial membrane potential.

Selected Publications

Priglinger E, Strasser J, Buchroithner B, Weber F, Wolbank S, Auer D, Grasmann E, Arzt C, Sivun D, Grillari J, Jacak J, Preiner J, Gimona M (2021) Label-free characterization of an extracellular vesicle-based therapeutic. J Extracell Vesicles. 2021 Oct;10(12):e12156.
(free PDF)

Weidinger A, Poženel L, Wolbank S, Banerjee A (2021). Sub-Regional Differences of the Human Amniotic Membrane and Their Potential Impact on Tissue Regeneration Application. Front Bioeng Biotechnol. 2021 Jan 13;8:613804.
(free PDF)

Priglinger E, Strohmeier K, Weigl M, Lindner C, Auer D, Gimona M, Barsch M, Jacak J, Redl H, Grillari J, Sandhofer M, Hackl M, Wolbank S (2020). SVF-derived extracellular vesicles carry characteristic miRNAs in lipedema. Sci Rep. 2020 Apr 29;10(1):7211.
(free PDF)

Poženel L, Lindenmair A, Schmidt K, Kozlov AV, Grillari J, Wolbank S, Banerjee A, Weidinger A (2019). Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro.Cells. 2019 Dec 15;8(12):1641.
(free PDF)

Nürnberger S, Lindner C, Maier J, Strohmeier K, Wurzer C, Slezak P, Suessner S, Holnthoner W, Redl H, Wolbank S, Priglinger E (2019). Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction. Eur Cell Mater. 2019 Feb 22;37:113-133. 

Priglinger E, Maier J, Chaudary S, Lindner C, Wurzer C, Rieger S, Redl H, Wolbank S, Dungel P (2018). Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application. J Tissue Eng Regen Med. 2018 Jun;12(6):1352-1362.

Banerjee A, Lindenmair A, Steinborn R, Dumitrescu SD, Hennerbichler S, Kozlov AV, Redl H, Wolbank S, Weidinger A (2018). Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro. Stem Cells Int. 2018 Oct 28;2018:9502451.
(free PDF)

Banerjee A, Lindenmair A, Hennerbichler S, Steindorf P, Steinborn R, Kozlov AV, Redl H, Wolbank S, Weidinger A (2018). Cellular and Site-Specific Mitochondrial Characterization of Vital Human Amniotic Membrane. Cell Transplant. 2018 Jan;27(1):3-11.
(free PDF)

Priglinger E, Schuh CMAP, Steffenhagen C, Wurzer C, Maier J, Nuernberger S, Holnthoner W, Fuchs C, Suessner S, Rünzler D, Redl H, Wolbank S (2017). Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy. Cytotherapy. 2017 Sep;19(9):1079-1095.

Priglinger E, Wurzer C, Steffenhagen C, Maier J, Hofer V, Peterbauer A, Nuernberger S, Redl H, Wolbank S, Sandhofer M (2017). The adipose tissue-derived stromal vascular fraction cells from lipedema patients: Are they different? Cytotherapy. 2017 Jul;19(7):849-860.

Banerjee A, Weidinger A, Hofer M, Steinborn R, Lindenmair A, Hennerbichler-Lugscheider S, Eibl J, Redl H, Kozlov AV, Wolbank S (2015) Different metabolic activity in placental and reflected regions of the human amniotic membrane. Placenta. 2015 Nov;36(11):1329-32.

Banerjee A, Nürnberger S, Hennerbichler S, Riedl S, Schuh CM, Hacobian A, Teuschl A, Eibl J, Redl H & Wolbank S (2014). In toto differentiation of human amniotic membrane towards the Schwann cell lineage. Cell Tissue Bank, 15(2):227-239.

Lindenmair A, Nürnberger S, Stadler G, Meinl A, Hackl C, Eibl J, Gabriel C, Hennerbichler S, Redl H & Wolbank S (2014). Intact human amniotic membrane differentiated towards the chondrogenic lineage. Cell Tissue Bank, 15(2):213-225.