Nanoparticle-based Therapies

The major research focus is the development of methods for quantitative analysis of biological systems at the nanoscale. Our group has long-time experience in single molecule fluorescence microscopy, force microscopy as well as 2D/3D nanolithography. By precisely localising and measuring the dynamics of biomolecules and determining molecular interactions, we seek to understand their function in model systems as well as in living cells. We are currently engaged in method development for the characterisation and understanding of physical properties of individual cells, nanobioparticles or lipids and proteins. Another important aspect of our work is the application of our research to the development of device technologies and image processing tools.

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Nanoscopy
Dr. Jaroslaw Jacak
Research Associate wnebfynj.wnpnx@genhzn.yot.np.ng
Dr. Birgit Plochberger
Research Associate ovetvg.cybpuoretre@genhzn.yot.np.ng

Selected Publications

Sych T, Schlegel J, Barriga HMG, Ojansivu M, Hanke L, Weber F, Beklem Bostancioglu R, Ezzat K, Stangl H, Plochberger B, Laurencikiene J, El Andaloussi S, Fürth D, Stevens MM, Sezgin E. (2023). High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler. Nat Biotechnol. 2023 Jun 12. 

Puthukodan S, Hofmann M, Mairhofer M, Janout H, Schurr J, Hauser F, Naderer C, Preiner J, Winkler S, Sivun D, Jacak J (2023). Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy. Anal Chem. 2023 Apr 11;95(14):6061-6070. 
(free PDF)

Strohmeier K, Hofmann M, Jacak J, Narzt MS, Wahlmueller M, Mairhofer M, Schaedl B, Holnthoner W, Barsch M, Sandhofer M, Wolbank S, Priglinger E. (2023). Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema. Biomedicines. 2022 May 18;10(5):1163.
(free PDF)

Puthukodan S, Hofmann M, Mairhofer M, Janout H, Schurr J, Hauser F, Naderer C, Preiner J, Winkler S, Sivun D, Jacak J. (2023). Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy. Anal Chem. 2023 Apr 11;95(14):6061-6070. 
(free PDF)

Strohmeier K, Hofmann M, Hauser F, Sivun D, Puthukodan S, Karner A, Sandner G, Le Renard PE, Jacak J, Mairhofer M (2021). CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis. Int J Mol Sci. 2021 Dec 28;23(1):282. 
(free PDF)

Priglinger E, Strasser J, Buchroithner B, Weber F, Wolbank S, Auer D, Grasmann E, Arzt C, Sivun D, Grillari J, Jacak J, Preiner J, Gimona M (2021). Label-free characterization of an extracellular vesicle-based therapeutic. J Extracell Vesicles. 2021 Oct;10(12):e12156.
(free PDF)